Proteomic has been very popular in research area when Western Blot was first described by Towbin et al in 1979. It has been also widely used in clinical diagnostic such as HIV test, or BSE test. Today, western blot is still widely accepted as one of the most powerful investigation tools in publication standards for cell signalling, immunology, and cancer researches.
Step
01
SAMPLE PREPARATION
Step
02
PROTEIN SEPARATION
Step
03
PROTEIN TRANSFER
Step
04
PROTEIN DETECTION & VISUALIZATION
Step 01 - Sample Preparation
There are many ways of sample preparation for proteomic, physical to chemical, from mild to aggressive, simple to complicated. Add on to that, Advansta offer range of product swhich can help the researcher to purify the protein lysate and enhance the quality of their research experience!
Step 02 - PROTEIN SEPARATION
SDS PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is a technique used to separate proteins based on their molecular weight. The method involves denaturing the proteins with SDS and then subjecting them to electrophoresis through a polyacrylamide gel. Advansta offers a range of products for SDS PAGE including running buffers and sample staining solutions. Meanwhile, Biometra offers a comprehensive range of polyacrylamide gel electrophoresis (PAGE) and blotting solutions for optimal proteins and nucleic acid analysis.
Protein Separation:
Sodium dodecyl Sulfate Polyacrylamide Gel Electrophoresist (SDS-PAGE)
Step 03 - PROTEIN TRANSFER
Protein transfer, also known as electroblotting, is a technique used to transfer proteins from a polyacrylamide gel onto a membrane for further analysis. The method involves the application of an electric field to drive the proteins out of the gel and onto the membrane. Advansta offers a range of transfer membranes, including nitrocellulose and PVDF, as well as transfer buffer and blocking solutions to optimize protein transfer efficiency. Analytik Jena provides a complete system for automated western blotting, including transfer, blocking, and detection steps, with integrated software for analysis and quantification of results.
Step 04 - PROTEIN DETECTION & VISUALIZATION
Protein detection and visualization is a crucial step in western blot analysis, allowing researchers to identify and quantify the proteins of interest. The process involves the use of specific antibodies that bind to the target protein, followed by the detection and visualization of the antibody-protein complex. Advansta offers a range of detection reagents, including horseradish peroxidase (HRP) and alkaline phosphatase (AP) substrates, as well as fluorescent and chemiluminescent detection options, all optimized for maximum sensitivity and signal-to-noise ratio. Aplegen Omega Lum series provides high-performance imaging systems, featuring advanced CCD cameras and software for quantitative analysis of western blot data, ensuring accurate and reliable results. Together, these products offer a comprehensive solution for protein detection and visualization in western blot analysis, delivering exceptional value for every penny invested.
