The innuPREP DOUBLEpure Kit was designed for efficient extraction of DNA fragments from TAE or TBE agarose gels and utilizes a novel 2-step technology for purifying amplification products from PCR reaction mixtures and sequencing reaction mixtures. The spin filter column has a binding capacity of over 20 µg, allowing for high yields and excellent quality when isolating DNA fragments. Recovery rates amount to 95 % depending on fragment size. Other features of the innuPREP DOUBLEpure Kit include easy handling, flexible choice of elution volume and fast purification protocols.
Average recovery rates
PCR-purification: ca. 60 % – 95 %
Gel extraction: ca. 60 % – 90 %
Sequencing reaction purification: > 75 %
depending on fragment length
Extraction time
PCR purification: approx. 3 minutes (2-step process)
Gel extraction: approx. 20 minutes
Sequencing reaction purification: approx. 2 minutes (2-step process)
Binding capacity
Column binding capacity: > 20 µg DNA
Fragment lengths
PCR-purification: > 60 bp – 30 kb
Gel extraction: 100 bp – 30 kb
Starting Material
PCR reaction mixes (up to 50 µl)
TAE agarose gels (up to 300 mg)
TBE agarose gels (up to 300 mg)
Sequencing reaction mixture (up to 50 µl)
| Product# | Product Name |
|---|---|
| IST#845-KS-5050050 | innuPREP DOUBLEpure Kit - 50 reactions |